LIPE-AS1

Species: Homo sapiens

Position: chr19: 42397147-42652355

Known as: LIPE-AS1 , ENSG00000213904

Transcript: NR_073179 , NR_073180 , ENST00000594688 , ENST00000457234 , ENST00000594624

Sequence: Download

Description:

LIPE-AS1 and CEACAM1 were one pair of inversely regulated upon DNA damage in the microarray data and validated by RT-qPCR. The lncRNA LIPE-AS1 was downregulated, while its antisense mRNA CEACAM1 was upregulated. Through generating knockdown cell lines for LIPE-AS1 as well as CEACAM1 using the dCas9-KRAB CRISPRi system, our data indicate that neither transcript- nor transcription-dependent mechanisms explain the inverse regulation of LIPEAS1:CEACAM1 or NOP14-AS1:NOP14 expression. Hence, sense-antisense pairs whose expression is strongly--positively or negatively--correlated can be nonetheless regulated independently.



sgRNAs

sgRNA_ID Sequence Position (Chr) Position (Lnc) Length PAM Type Validity Cell line Note Ref.
sgRNA1 GCCTATCGCTGCCCGAGTT 42397072-42397090(-) up stream 19 GGG CRISPRi Experimental validated A549 NA [1]
sgRNA2 GGGCCTCTGCGGAGTGGACC 42397164-42397183(-) gene body (near 5') 20 AGG CRISPRi Experimental validated A549 NA [1]

GBrowser


Links

lncRNA Function:

sgRNA Design Tool:

Reference

1. Goyal A, Fiškin E, Gutschner T, Polycarpou-Schwarz M, Groß M, et al. (2017). A cautionary tale of sense-antisense gene pairs: independent regulation despite inverse correlation of expression. Nucleic Acids Res 45(21): 12496-12508.