Species: Homo sapiens

Position: chr11: 65497687-65506516

Known as: MALAT1 , ENSG00000251562

Transcript: NR_002819 , NR_144567 , NR_144568 , ENST00000534336 , ENST00000619449

Sequence: Display Download

Description:

MALAT1 (metastasis associated lung adenocarcinoma transcript 1) is upregulated in many cancers, such as breast, pancreatic, lung, colon, prostate and liver cancer, and could inhibit tumor cell apoptosis and promote cell invasion in bladder cancer. Using "CRISPR Activation" (CRISPRa) and "CRISPR Interference" (CRISPRi) to up- and down-regulate MALAT1 expression, a reciprocal repression feedback loop between miR-125b and MALAT1 was found. Besides, due to the high expression in a variety of cell lines, MALAT1 is frequently used as representative lncRNA to develop CRISPR-cas9 techniques, like Crispr/Cas9-based HDR-mediated biallelic integration of poly(A), paired gRNA-guided CRISPR/Cas9 knockout (paired-KO) method, Double Excision CRISPR Knockout (DECKO), etc.

1. Xie H, Liao X, Chen Z, Fang Y, He A, et al. (2017). LncRNA MALAT1 Inhibits Apoptosis and Promotes Invasion by Antagonizing miR-125b in Bladder Cancer Cells. J Cancer 8(18): 3803-3811.

2. Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, et al. (2014). Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell 159(3): 647-61.

3. Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigó R, et al. (2015). DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. BMC Genomics 16: 846.

4. Liu Y, Han X, Yuan J, Geng T, Chen S, et al. (2017). Biallelic insertion of a transcriptional terminator via the CRISPR/Cas9 system efficiently silences expression of protein-coding and non-coding RNA genes. J Biol Chem 292(14): 5624-5633.

5. Janga H, Aznaourova M, Boldt F, Damm K, Grünweller A, et al. (2018). Cas9-mediated excision of proximal DNaseI/H3K4me3 signatures confers robust silencing of microRNA and long non-coding RNA genes. PLoS One 13(2): e0193066.

6. Liu Z, Hui Y, Shi L, Chen Z, Xu X, et al. (2016). Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells. Stem Cell Reports 7(3): 496-507.

7. Stojic L, Lun ATL, Mangei J, Mascalchi P, Quarantotti V, et al. (2018). Specificity of RNAi, LNA and CRISPRi as loss-of-function methods in transcriptional analysis. Nucleic Acids Res.

8. Kim J, Piao HL, Kim BJ, Yao F, Han Z, et al. (2018). Long noncoding RNA MALAT1 suppresses breast cancer metastasis. Nat Genet 50(12): 1705-1715.

9. Zhu S, Li W, Liu J, Chen CH, Liao Q, et al. (2016). Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library. Nat Biotechnol 34(12): 1279-1286.

10. Qu D, Sun WW, Li L, Ma L, Sun L, et al. (2019). Long noncoding RNA MALAT1 releases epigenetic silencing of HIV-1 replication by displacing the polycomb repressive complex 2 from binding to the LTR promoter. Nucleic Acids Res 47(6): 3013-3027.