TTTY15
Species: Homo sapiens
Position: chrY: 12662322-12692233
Known as: NCRNA00138 , ENSG00000114374
Transcript: NR_001545 , XR_002958839 , XR_002958838 , ENST00000440408
Sequence: Download
Description:
TTTY15 was the most elevated lncRNA located on the Y chromosome, In most patients with PCa. Knockout of this lncRNA by two different CRISPRCas9 strategies suppressed PCa cell growth both in vitro and in vivo. TTTY15 promoted PCa by sponging the microRNA let-7, consequently increasing CDK6 and FN1 expression. FOXA1 is an upstream regulatory factor of TTTY15 transcription.
sgRNAs
| sgRNA_ID | Sequence | Position (Chr) | Position (Lnc) | Length | PAM | Type | Validity | Cell line | Note | Ref. |
|---|---|---|---|---|---|---|---|---|---|---|
| sgRNA1 | TCAGGCAATCCAGCCGCCC | 12664914-12664932(-) | gene body (near 5') | 19 | GGG | CRISPRko/ki | High activity | DU145 | paried with sgRNA2, We successfully obtained four TTTY15-KO cell clones from 10 selected single-cell clones | [1] |
| sgRNA2 | CGAGCTTGAAACTAAGAGC | 12701854-12701872(+) | down stream | 19 | TGG | CRISPRko | High activity | DU145 | paried with sgRNA1, We successfully obtained four TTTY15-KO cell clones from 10 selected single-cell clones | [1] |
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Reference
1. Xiao G, Yao J, Kong D, Ye C, Chen R, et al. (2019). The Long Noncoding RNA TTTY15, Which Is Located on the Y Chromosome, Promotes Prostate Cancer Progression by Sponging let-7. Eur Urol 76(3): 315-326.
